fUSE5
Filamentous phage display vector fUSE5
The fUSE5 phage display vector is derived by insertion of a synthetic 14 bp stuffer sequence into gene III of the filamentous phage vector fd-tet (AF217317.1). The frameshifting stuffer must be replaced with a frame-restoring peptide insert to express pIII and enable
E. coli infectivity. The fUSE5 vector can be used to create new phage display peptide libraries by replacing the 14 bp stuffer with random oligonucleotides.
Product Specifications
| Product | Bacteriophage display vector |
|---|---|
| Cat. Number | COV0002 |
| Genbank | AF218364.1 |
| Sequence | >AF218364.1 |
| Size | 9206 bp |
| Insert | 14 bp stuffer |
| Bacterial resistance | Tetracycline (20 μg/mL) |
| Growth strains | K91BluKan |
| Reference | Scott JK et al. Science. 1990 Jul 27;249(4967):386-90. doi: 10.1126/science. |
| Price | $195.00 |
Please Cite
The fUSE5 vector was created by Dr. George Smith at the University of Missouri and is distributed by Cell Origins (#COV0002).
References section:
Scott JK, Smith GP. Searching for peptide ligands with an epitope library. Science. 1990 Jul 27;249(4967):386-90. doi: 10.1126/science.1696028.