Wash Buffer
Wash Buffer (100 mL)
The wash buffer (0.5% Tween-20 in TBS) is the standard used most phage-biomolecular interactions studies such as in micropanning experiments, ELISA, and fluorescent microscopy. The concentration of Tween-20 may be adjusted to change the stringency of the washing.
Reagents
- 0.5 mL Tween-20
-
TBS
Protocol
- Mix 0.5 mL Tween-20 with 99.5 mL TBS.
- The solution may be filter sterilized, although this is not necessary if stored at 4°C and used within a few weeks.