f88-4

Filamentous phage display vector f88-4

The f88-4 phage display vector is based on the filamentous phage cloning vector fd-tet (AF217317.1). The vector contains two major coat protein genes (wild-type gene VIII and artificial gene rVIII) resulting in the expression of the two proteins under the induction of IPTG. The artificial gene rVIII contains a 21 bp stuffer that can be replaced with random oligonucleotides to create a phage display library.

Product Specifications

Bacteriophage display vector
Cat. Number COV0004
Genbank AF218363.1
Sequence >AF218363.1
Size 9234 bp (dsDNA)
Insert 21 bp stuffer
Bacterial resistance Tetracycline (20 μg/mL)
Growth strains K91BluKan
Reference Scott JK, et al. Science. 1990; 249(4967):386-90
Price $195.00

Please Cite

The f88-4 vector was created by Dr. George Smith at the University of Missouri and is distributed by Cell Origins (#COV0004).


References section:

Scott JK, Smith GP. Searching for peptide ligands with an epitope library. Science. 1990 Jul 27;249(4967):386-90. doi: 10.1126/science.1696028.

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