E. COLI STRAINS

E. coli Strains

The filamentous bacteriophage viruses (M13, fd, and f1) infect gram-negative bacteria. Of these, Escherichia coli ( E. coli ) is the most commonly used in phage display technology. The filamentous bacteriophages infect E. coli by binding via coat protein III to the F-pilus of the bacterial cell. When the F-pilus comes into contact with pIII, the pilus retracts, allowing the genetic material of the phage to be delivered into the host cell. The fd and M13 species of bacteriophages share similar biology; they both release viral progeny through the plasma membrane of E. coli without causing cell lysis. This characteristic makes it easy to propagate filamentous bacteriophages in the lab, making them highly suitable for various phage display selection techniques.

Cell Origins provides the most commonly used E. coli strains used in both bacteriophage and phagemid-based phage display technology.

E. coli Strains