f3TR1 cyclic peptide phage display library (14-mer)
Cell Origins provides researchers with critical phage-based laboratory services and kits. We focus on the use of filamentous phage as an inexpensive, reliable, highly stable, and effective replacement for many products currently on the market.
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The f3TR1 14-mer cyclic peptide phage display library is built from the
f3TR1 filamentous phage vector (HM355479.1) by replacing a synthetic 14 bp stuffer sequence with frame-restoring 42 bp random oligo nucleotides. The expressed peptides contains cysteines at positions 3 and 12 (XXCXXXXXXXXCXX) constraining the peptide in a cyclic conformation under oxidative conditions. The 14-mer foreign peptide insert is expressed on pIII as a fusion to the N-terminus of pIII through a trypsin-recognition sequence. The f3TR1 phage display library maintains
E. coli infectivity and does not require a helper phage for assembly and release of virions.
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Please Cite
The f3TR1 vector was created by Dr. George Smith at the University of Missouri and the f3TR1 cyclic 14-mer library was created by Cell Origins (#COPD0003).
References section:
Thomas WD, Golomb M, Smith GP. Corruption of phage display libraries by target-unrelated clones: diagnosis and countermeasures. Anal Biochem. 2010 Dec 15;407(2):237-40. doi: 10.1016/j.ab.2010.07.037.