1x Tris-Buffered Saline (TBS; 1 L)
Cell Origins provides researchers with critical phage-based laboratory services and kits. We focus on the use of filamentous phage as an inexpensive, reliable, highly stable, and effective replacement for many products currently on the market.
Complete page context
Tris-buffered saline (TBS) is widely utilized as a versatile wash and dilution solution in various applications, such as phage display, cell culture, Western blotting, and enzyme-linked immunosorbent assay (ELISA). It provides an optimal balance of buffering capacity and ionic strength, ensuring accurate and reliable results in biological and biochemical experiments. TBS is the go-to buffer for most phage-related protocols including biopanning, phage purification, and storage. The final concentrations of Tris-HCl and NaCl in this protocol are 50 mM and 150 mM, respectively.
Reagents
- 6.05 g Tris-HCl
- 8.76 g NaCl
Protocol
- Dissolve 6.05 g Tris-HCl and 8.76 g NaCl in 800 mL of H2O.
- Adjust the pH to 7.5 with 1 M HCl and add H2O to a final volume of 1 L.
- Autoclave and store at room temperature.