Standard solution

Wash Buffer (100 mL)

Cell Origins provides researchers with critical phage-based laboratory services and kits. We focus on the use of filamentous phage as an inexpensive, reliable, highly stable, and effective replacement for many products currently on the market.

Recovered detail

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The wash buffer (0.5% Tween-20 in TBS) is the standard used most phage-biomolecular interactions studies such as in micropanning experiments, ELISA, and fluorescent microscopy. The concentration of Tween-20 may be adjusted to change the stringency of the washing.

Reagents

- 0.5 mL Tween-20

- TBS

Protocol

- Mix 0.5 mL Tween-20 with 99.5 mL TBS.

- The solution may be filter sterilized, although this is not necessary if stored at 4°C and used within a few weeks.