Strain information
E. coli CJ236
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Recovered detail
Complete page context
E. coli CJ236 cells generate ssDNA for site-directed mutagenesis using the Kunkel method. These cells replace a portion of thymine(T) in their DNA with deoxyuracil(dU). The resulting ssDNA can be effectively utilized for mutagenesis. CJ236 is dut- ung- and carries an F-factor with a chloramphenicol resistance selectable marker.
Product Specifications
Please Cite
Vieira J, Messing J. Production of single-stranded plasmid DNA. Methods Enzymol. 1987;153:3-11. doi: 10.1016/0076-6879(87)53044-0.